The Acetyl-Histone H2B-K20 Polyclonal Antibody (CAB24886) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is raised in rabbits and is highly specific to acetylated histone H2B at lysine 20 in human samples. It has been validated for use in Western blot applications, allowing for the detection and analysis of this histone modification in various cell types.Histone acetylation at lysine 20 on H2B is known to play a role in gene expression regulation, chromatin structure, and cell differentiation. Aberrant acetylation at this site has been implicated in various diseases, including cancer and neurological disorders.
This antibody is validated for use in WB, ChIP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Acetyl-Histone H2B-K20 Polyclonal Antibody
SKU:
CAB24886
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBChIPELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
HeLa treated with TSA, NIH/3T3 treated with TSA, C6 treated with TSA
Cellular Localization:
Chromosome, Nucleus.
Calculated MW:
14kDa
Observed MW:
17kDa
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-dependent histone that is a member of the histone H2B family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. The protein has antibacterial and antifungal antimicrobial activity.
Purification Method
Affinity purification
Gene ID
3017 8349
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Acetyl-Histone H2B-K20 Rabbit pAb (CAB24886) at 1:500 dilution. HeLa cells,NIH/3T3 cells and C6 cells were treated with TSA (1 μM) at 37℃ for 18 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.
Chromatin immunoprecipitation was performed with cross-linked chromatin from 293F cells, using Acetyl-Histone H2B-K20 Rabbit pAb (CAB24886) and rabbit IgG (AC005). The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram compares the ratio of the immunoprecipitated DNA versus the input.
_WB_02__03132.1701347421.jpg?c=2?imbypass=on "Western blot analysis of various lysates using Acetyl-Histone H2B-K20 Rabbit pAb (CAB24886) at 1:500 dilution.  HeLa cells, NIH/3T3 cells and C6 cells were treated by TSA (1 μM) at 37℃ for 18 hours. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit (RM00020). Exposuretime: 20s.")
_WB_02__03132.1701347421.jpg?c=2 "Western blot analysis of various lysates using Acetyl-Histone H2B-K20 Rabbit pAb (CAB24886) at 1:500 dilution.  HeLa cells, NIH/3T3 cells and C6 cells were treated by TSA (1 μM) at 37℃ for 18 hours. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit (RM00020). Exposuretime: 20s.")
