The Acetyl-Histone H4-K16 Antibody (CAB5280) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly reactive with acetylated histone H4 at lysine 16 in human samples, making it a valuable tool for epigenetics and gene regulation studies.Histone acetylation at lysine 16 of histone H4 is known to be involved in gene activation and chromatin remodeling. This modification plays a crucial role in regulating gene expression and can impact various cellular processes, including proliferation, differentiation, and DNA repair.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat, Other (Wide Range Predicted) samples.
Product Name:
Acetyl-Histone H4-K16 Antibody
SKU:
CAB5280
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat, Other (Wide Range Predicted)
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
GGAK R
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:100 - 1:500
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.
Purification Method
Affinity purification
Gene ID
8359
RRID
AB_2766099
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using Acetyl-Histone H4-K16 Rabbit pAb (CAB5280) at 1:400 dilution. NIH/3T3 and C6 cells were treated with TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of C6 cells treated with TSA (upper left) and untreated C6 cells (upper right) using Acetyl-Histone H4-K16 Rabbit pAb (red, CAB5280) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H4-K16 (CAB5280) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007' target='_blank' >AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.NIH/3T3 cells were treated with TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007' target='_blank' >AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using Acetyl-Histone H4-K16 (CAB5280) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007' target='_blank' >AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.U2OS cells were treated with TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007' target='_blank' >AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
