The ACOX2 Antibody (CAB12796) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced in rabbits and exhibits high reactivity with human samples, making it ideal for use in Western blot applications. By binding specifically to the ACOX2 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it a valuable asset for studies in metabolism, lipid biology, and related research areas.ACOX2, also known as peroxisomal acyl-coenzyme A oxidase 2, plays a crucial role in lipid metabolism by catalyzing the breakdown of fatty acids into smaller molecules.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
ACOX2 Antibody
SKU:
CAB12796
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BCOX, BRCOX, CBAS6, THCCox, BRCACOX, ACOX2
Positive Sample:
293T, 293T-ACOX2-N, Mouse kidney
Cellular Localization:
Peroxisome.
Calculated MW:
77kDa
Observed MW:
76kDa
The product of this gene belongs to the acyl-CoA oxidase family. It encodes the branched-chain acyl-CoA oxidase which is involved in the degradation of long branched fatty acids and bile acid intermediates in peroxisomes. Deficiency of this enzyme results in the accumulation of branched fatty acids and bile acid intermediates, and may lead to Zellweger syndrome, severe cognitive disability, and death in children.
Purification Method
Affinity purification
Gene ID
8309
RRID
AB_2759635
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using ACOX2 Rabbit pAb (CAB12796) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of lysates from Mouse kidney, using ACOX2 Rabbit pAb (CAB12796) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
