In a similar fashion, the Akt/PKB Polyclonal Antibody (CAB6239) is an essential tool for researchers studying the Akt protein, a key player in the PI3K signaling pathway involved in cell growth, survival, and metabolism. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to Akt, this antibody allows for the precise detection and analysis of Akt protein levels in a variety of cell types.Akt, also known as Protein Kinase B (PKB), is a crucial regulator of cell proliferation and survival, making it a promising target for research in cancer biology, diabetes, and other diseases associated with aberrant cell signaling.
Understanding the activity and regulation of Akt is essential for developing targeted therapies that modulate cell growth and survival pathways in pathological conditions.Overall, the Akt/PKB Polyclonal Antibody (CAB6239) offers researchers a valuable tool for studying the role of Akt in various cellular processes and diseases, ultimately contributing to the advancement of medical research and the development of novel therapeutic strategies.
Product Name:
AKTIP Rabbit Polyclonal Antibody
SKU:
CAB6239
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-292 of human AKTIP (NP_071921.1).
The mouse homolog of this gene produces fused toes and thymic hyperplasia in heterozygous mutant animals while homozygous mutants die in early development. This gene may play a role in apoptosis as these morphological abnormalities are caused by altered patterns of programmed cell death. The protein encoded by this gene is similar to the ubiquitin ligase domain of other ubiquitin-conjugating enzymes but lacks the conserved cysteine residue that enables those enzymes to conjugate ubiquitin to the target protein. This protein interacts directly with serine/threonine kinase protein kinase B (PKB)/Akt and modulates PKB activity by enhancing the phosphorylation of PKB's regulatory sites. Alternative splicing results in two transcript variants encoding the same protein.
Purification Method:
Affinity purification
Gene ID:
64400
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using AKTIP Rabbit pAb (CAB6239) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 40s.
