The ALDH1B1 Antibody (CAB3725) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has high reactivity with human samples and has been validated for use in Western blot applications. It specifically binds to the ALDH1B1 protein, allowing for accurate detection and analysis in a variety of cell types.ALDH1B1 is known for its involvement in the oxidation of aldehydes, playing a role in protecting cells from oxidative stress and maintaining cellular health. Research into ALDH1B1 is important for understanding its function in various biological processes and its potential implications in diseases such as cancer, neurodegenerative disorders, and metabolic conditions.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ALDH1B1 Antibody
SKU:
CAB3725
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ALDH5, ALDHX, ALDH1B1
Positive Sample:
SW620, HepG2, SKOV3, Mouse testis, Mouse heart, Rat liver
Cellular Localization:
Mitochondrion Matrix.
Calculated MW:
57kDa
Observed MW:
57kDa
This protein belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. This gene does not contain introns in the coding sequence. The variation of this locus may affect the development of alcohol-related problems.
Purification Method
Affinity purification
Gene ID
219
RRID
AB_2765232
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ALDH1B1 Rabbit pAb (CAB3725) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunofluorescence analysis of HeLa cells using ALDH1B1 Rabbit pAb (CAB3725) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
