The ALKBH3 Antibody (CAB5808) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is suitable for use in Western blot applications. It specifically targets the ALKBH3 protein, enabling precise detection and analysis in various cell types.ALKBH3 is known for its role in repairing DNA damage caused by alkylating agents and is also implicated in protecting cells from oxidative stress.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
ALKBH3 Antibody
SKU:
CAB5808
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ABH3, PCA1, DEPC1, hABH3, DEPC-1, ALKBH3
Positive Sample:
Mouse heart
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
33kDa
Observed MW:
33kDa
The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 (MIM 610602) and ALKBH3 are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine (Duncan et al., 2002 [PubMed 12486230]).
Purification Method
Affinity purification
Gene ID
221120
RRID
AB_2766560
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse heart, using ALKBH3 Rabbit pAb (CAB5808) at 1:4000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
