Anti-Alpha-2-antiplasmin Antibody (CAB9945)

Antibody Name:	Anti-Alpha-2-antiplasmin Antibody
Antibody SKU:	CAB9945
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 302-491 of human SERPINF2 (NP_001159392.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Rat
Positive Samples:	HepG2, A-549, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 302-491 of human SERPINF2 (NP_001159392.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	VPTH FEWN VSQV LANL SWDT LHPP LVWE RPTK VRLP KLYL KHQM DLVA TLSQ LGLQ ELFQ APDL RGIS EQSL VVSG VQHQ STLE LSEV GVEA AAAT SIAM SRMS LSSF SVNR PFLF FIFE DTTG LPLF VGSV RNPN PSAP RELK EQQD SPGN KDFL QSLK GFPR GDKL FGPD LKLV PPME EDYP QFGS PK
Gene ID:	5345
Uniprot:	P08697
Cellular Location:	Secreted
Calculated MW:	47kDa/54kDa
Observed MW:	65kDa

Synonyms:

SERPINF2, A2AP, AAP, ALPHA-2-PI, API, PLI

Background:

This gene encodes a member of the serpin family of serine protease inhibitors. The protein is a major inhibitor of plasmin, which degrades fibrin and various other proteins. Consequently, the proper function of this gene has a major role in regulating the blood clotting pathway. Mutations in this gene result in alpha-2-plasmin inhibitor deficiency, which is characterized by severe hemorrhagic diathesis. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	SERPINF2: Serine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin. Defects in SERPINF2 are the cause of alpha-2-plasmin inhibitor deficiency (APLID). APLID is an autosomal recessive disorder resulting in severe hemorrhagic diathesis. Belongs to the serpin family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 17p13 Cellular Component: extracellular space; cell surface; fibrinogen complex; extracellular region Molecular Function:serine-type endopeptidase inhibitor activity; protein binding; protein homodimerization activity; protease binding; endopeptidase inhibitor activity Biological Process: platelet activation; collagen fibril organization; positive regulation of smooth muscle cell proliferation; positive regulation of collagen biosynthetic process; positive regulation of JNK cascade; response to organic substance; fibrinolysis; negative regulation of fibrinolysis; platelet degranulation; renin-angiotensin regulation of blood vessel size; positive regulation of stress fiber formation; acute-phase response; blood vessel morphogenesis; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; positive regulation of cell differentiation Disease: Alpha-2-plasmin Inhibitor Deficiency
NCBI Summary:	This gene encodes a member of the serpin family of serine protease inhibitors. The protein is a major inhibitor of plasmin, which degrades fibrin and various other proteins. Consequently, the proper function of this gene has a major role in regulating the blood clotting pathway. Mutations in this gene result in alpha-2-plasmin inhibitor deficiency, which is characterized by severe hemorrhagic diathesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
UniProt Code:	P08697
NCBI GenInfo Identifier:	260064048
NCBI Gene ID:	5345
NCBI Accession:	NP_001159392.1
UniProt Secondary Accession:	P08697,Q8N5U7, Q9UCG2, Q9UCG3, B4E1B7,
UniProt Related Accession:	P08697
Molecular Weight:	47,907 Da
NCBI Full Name:	alpha-2-antiplasmin isoform a
NCBI Synonym Full Names:	serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2
NCBI Official Symbol:	SERPINF2
NCBI Official Synonym Symbols:	AAP; API; PLI; A2AP; ALPHA-2-PI
NCBI Protein Information:	alpha-2-antiplasmin; alpha-2-AP; alpha-2-plasmin inhibitor; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2; serpin F2
UniProt Protein Name:	Alpha-2-antiplasmin
UniProt Synonym Protein Names:	Alpha-2-plasmin inhibitor; Alpha-2-PI; Serpin F2
UniProt Gene Name:	SERPINF2
UniProt Entry Name:	A2AP_HUMAN

	Western blot analysis of extracts of various cell lines, using SERPINF2 antibody (CAB9945) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded rat pancreas using SERPINF2 antibody (CAB9945) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-SERPINF2 Antibody (CAB14043)	Human alpha2-AP ( alpha2-Antiplasmin) ELISA Kit (HUES02090)
SERPINF2 Antibody (PACO12120)	Human Alpha-2-antiplasmin (SERPINF2) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)