The THOC4/ALYREF Monoclonal Antibody (CAB4298) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is a heat stable, nuclear protein and functions as a molecular chaperone. It is thought to regulate dimerization, DNA binding, and transcriptional activity of basic region-leucine zipper (bZIP) proteins.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
THOC4/ALYREF Monoclonal Antibody
SKU:
CAB4298
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0959
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:500 - 1:1000
IHC-P
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ALY, BEF, REF, THOC4, ALY/REF, THOC4/ALYREF
Positive Sample:
HeLa, 293T, A-549, Mouse brain
Cellular Localization:
Nucleus. Nucleus Speckle. Cytoplasm. Colocalizes With The Core Ejc, Thoc4, Nxf1 And Ddx39B In The Nucleus And Nuclear Speckles. Travels To The Cytoplasm As Part Of The Exon Junction Complex (Ejc) Bound To Mrna.
Calculated MW:
27kDa
Observed MW:
32kDa
The protein encoded by this gene is a heat stable, nuclear protein and functions as a molecular chaperone. It is thought to regulate dimerization, DNA binding, and transcriptional activity of basic region-leucine zipper (bZIP) proteins.
Purification Method
Affinity purification
Gene ID
10189
RRID
AB_2863229
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using THOC4/ALYREF Rabbit mAb (CAB4298) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Mouse heart tissue using THOC4/ALYREF Rabbit mAb (CAB4298) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using THOC4/ALYREF Rabbit mAb (CAB4298) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using THOC4/ALYREF Rabbit mAb (CAB4298) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using THOC4/ALYREF Rabbit mAb (CAB4298) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
