Ammonia Assay Kit - Information
Assay Genie's ammonia assay is based on an improved o-phthalaldehyde method. This reagent reacts with ammonia/ ammonium and forms a fluorescent product. The fluorescence intensity (Ex/Em = 360/450nm) is proportional to the ammonia concentration in the sample.
Applications
For quantitative determination of ammonia/ammonium concentration by fluorimetry at 360nm/450nm
Ammonia Assay Kit - Key Features
- Fast and sensitive. Linear detection range of 0.012 - 1 mM ammonia.
- Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Ammonia Assay Kit - Data Sheet | |
| Kit Includes | Assay Buffer: 20 mL Standard: 400 mL NH4Cl Reagent A: 1 mL Reagent B: 1 mL |
| Kit Requires | Pipetting devices, centrifuge tubes, black flat bottom 96-well plates and plate reader. |
| Method of Detection | FL360/450nm |
| Detection Limit | 12 |
| Samples | Biological (eg urine) and enviromental samples |
| Species | All |
| Protocol Length | 15 min |
| Size | 200 tests |
| Storage | Store kit at -20°C. |
| Shelf Life | 6 months |
More Details
AMMONIA (NH
3) or its ion form ammonium (NH
4+) is an important source of nitrogen for living systems and is ubiquitously present in the nature. Simple, direct and automation-ready procedures for measuring NH
3 are very desirable.
