The Anti-2-O-Methylguanosine (2-OMeG) Antibody (CAB20697) is a powerful tool for researchers studying RNA modifications and their impact on gene expression and cellular functions. This polyclonal antibody, raised in rabbits, specifically recognizes 2-OMeG, a common modification found in various types of RNA molecules.With its high reactivity towards 2-OMeG in human samples, this antibody is suitable for a range of applications, including immunofluorescence, immunohistochemistry, and ELISA. It allows for the visualization and detection of 2-OMeG in different cellular contexts, providing valuable insights into the role of RNA modifications in biological processes.
The study of RNA modifications, such as 2-OMeG, is a rapidly growing field with implications for various diseases, including cancer and neurological disorders. By using the Anti-2-O-Methylguanosine Antibody, researchers can further explore the functional significance of RNA modifications and their potential as targets for therapeutic interventions.
Product Name:
2'-O-Methylguanosine(Gm) Polyclonal Antibody
SKU:
CAB20697
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Species independent
Immunogen:
Gm
Sequence:
Gm c onju gate d to KLH
Tested Applications:
ELISADB
Recommended Dilution:
DB,1:500 - 1:2000
Conjugate:
Unconjugated
Observed MW:
Refer to figures
RNA methylation plays a significant regulatory role in various of physiological activities and it has gradually become a hotspot of epigenetics in the past decade. 2'-O-methyladenosine (Am), 2'-O-methylguanosine (Gm),2'-O-methylcytidine (Cm),2'-O-methyluridine (Um),N 6-methyladenosine (m6A), N 1-methylguanosine (m1G),5-methylcytidine (m5C),and 5-methyluridine (m5U) are representative 2'-O-methylation and base-methylation modified epigenetic marks of RNA. 2'-O-methyltransferase is a modified nucleoside that is produced in tRNAs by the action of tRNA guanosine-2'-O-methyltransferase, using S-adenosyl-L-methionine as a substrate. Through its interaction with other modified nucleosides, 2'-O-methylguanosine is thought to stabilize the structure of the tRNA.
Purification Method:
Affinity purification
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
The 2'-O-methylguanosine(Gm) Rabbit pAb(CAB20697) are tested in Dot Blot against 2'-O-methylguanosine(Gm) and unmodified guanosine. Gm-3：5'Biotin-GCATAATGACTACT(Gm)3' Gm-4：5'Biotin-GCATAATGACTACTG3'
