The 2'-O-methyluridine(Um) Monoclonal Antibody (CAB20695) is a high-quality antibody developed for reliable detection and analysis of target proteins. Ribose methylation is among the most ubiquitous modifications found in RNA. 2'-O-methyluridine is found in rRNA, snRNA, snoRNA and tRNA of Archaea, Bacteria, and Eukaryota. Moreover, 2'-O-methylribonucleosides are promising starting materials for the production of nucleic acid-based drugs. The chemical modification 2'-O-methyl of nucleosides is often used to increase siRNA stability towards nuclease activities.
This antibody is validated for use in ELISA, DB applications and has demonstrated reactivity against Species independent samples.
Product Name:
2'-O-methyluridine(Um) Monoclonal Antibody
SKU:
CAB20695
Size:
100μL, 20μL
Reactivity:
Species independent
Clone Number:
ARC50750
Conjugate:
Unconjugated
Immunogen:
Chemical compounds corresponding to 2'-O-methyluridine(Um).
Tested Applications:
ELISADB
Recommended Dilution:
DB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
Um, 2'-O-methyluridine, 2'-O-methyluridine(Um)
Observed MW:
Refer to figures
Ribose methylation is among the most ubiquitous modifications found in RNA. 2'-O-methyluridine is found in rRNA, snRNA, snoRNA and tRNA of Archaea, Bacteria, and Eukaryota. Moreover, 2'-O-methylribonucleosides are promising starting materials for the production of nucleic acid-based drugs. The chemical modification 2'-O-methyl of nucleosides is often used to increase siRNA stability towards nuclease activities.
Purification Method
Affinity purification
Gene ID
CAS: 2140-76-3
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Dot-blot analysis of 2'-O-methyluridine(Um) and unmodified uridine using 2'-O-methyluridine(Um) Rabbit mAb antibody (CAB20695) at 1:1000 dilution. 2'-O-methyluridine : Biotin-5'UGACAACUACAGAC(Um)3' unmodified uridine : Biotin-5'UGACAACUACAGACU3'
