The 2'-O-methyluridine(Um) Monoclonal Antibody (CAB20695) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, produced in rabbits, specifically targets the 2'-O-Methyluridine (UM) modification, allowing for precise detection and analysis of UM-containing RNA molecules. Validated for use in various applications such as immunoprecipitation and RNA sequencing, this antibody offers high specificity and sensitivity in identifying UM-modified RNAs.The 2'-O-Methyluridine modification is known to play important roles in RNA stability, translation efficiency, and RNA-protein interactions.
This antibody is validated for use in ELISA, DB applications and has demonstrated reactivity against Species independent samples.
Product Name:
2'-O-methyluridine(Um) Monoclonal Antibody
SKU:
CAB20695
Size:
20μL, 100μL
Reactivity:
Species independent
Clone Number:
ARC50750
Conjugate:
Unconjugated
Immunogen:
Chemical compounds corresponding to 2'-O-methyluridine(Um).
Sequence:
Um c onju gate d to KLH
Tested Applications:
ELISADB
Recommended Dilution:
DB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
Um, 2'-O-methyluridine, 2'-O-methyluridine(Um)
Observed MW:
Refertofigures
Ribose methylation is among the most ubiquitous modifications found in RNA. 2'-O-methyluridine is found in rRNA, snRNA, snoRNA and tRNA of Archaea, Bacteria, and Eukaryota. Moreover, 2'-O-methylribonucleosides are promising starting materials for the production of nucleic acid-based drugs. The chemical modification 2'-O-methyl of nucleosides is often used to increase siRNA stability towards nuclease activities.
Purification Method
Affinity purification
Gene ID
CAS: 2140-76-3
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Dot-blot analysis of 2'-O-methyluridine(Um) and unmodified uridine using 2'-O-methyluridine(Um) Rabbit mAb antibody (CAB20695) at 1:1000 dilution. 2'-O-methyluridine : Biotin-5'UGACAACUACAGAC(Um)3' unmodified uridine : Biotin-5'UGACAACUACAGACU3'
