The AhR Monoclonal Antibody (CAB4000) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody is produced using a polyclonal method in rabbits and has been validated for use in Western blotting, immunohistochemistry, and immunofluorescence assays.The AHR protein is a transcription factor that plays a crucial role in mediating the cellular response to environmental toxins and pollutants. Activation of AHR can lead to the regulation of various genes involved in detoxification and immune response pathways.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
AhR Monoclonal Antibody
SKU:
CAB4000
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC53212
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
The protein encoded by this gene is a ligand-activated helix-loop-helix transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. This receptor has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Before ligand binding, the encoded protein is sequestered in the cytoplasm; upon ligand binding, this protein moves to the nucleus and stimulates transcription of target genes.
Purification Method
Affinity purification
Gene ID
196
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using AHR Rabbit mAb (CAB4000) at 1:6000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC): Jurkat Exposure time: 90 s.
