The AP2S1 Monoclonal Antibody (CAB9263) is a high-quality antibody developed for reliable detection and analysis of target proteins. One of two major clathrin-associated adaptor complexes, AP-2, is a heterotetramer which is associated with the plasma membrane. This complex is composed of two large chains, a medium chain, and a small chain. This gene encodes the small chain of this complex. Alternative splicing results in multiple transcript variants.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
AP2S1 Monoclonal Antibody
SKU:
CAB9263
Size:
100μL, 20μL
Reactivity:
Mouse
Clone Number:
ARC1500
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
AP17, FBH3, HHC3, FBHOk, CLAPS2, AP2S1
Positive Sample:
Mouse brain
Cellular Localization:
Clathrin-Coated Endocytic Vesicle, Cytoplasmic Side Of Plasma Membrane, Cytosol, Plasma Membrane, Postsynapse, Presynapse.
Calculated MW:
17kDa
Observed MW:
17kDa
One of two major clathrin-associated adaptor complexes, AP-2, is a heterotetramer which is associated with the plasma membrane. This complex is composed of two large chains, a medium chain, and a small chain. This gene encodes the small chain of this complex. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
1175
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse brain , using AP2S1 Rabbit mAb (CAB9263) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
