The ASB17 Antibody (CAB20238) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits high specificity and sensitivity when detecting ASB17 in human samples, making it suitable for Western blot applications.ASB17 is known to play a role in the regulation of protein degradation and turnover, making it a key player in cellular homeostasis and signaling pathways.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
ASB17 Antibody
SKU:
CAB20238
Size:
20μL, 100μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
GHRF ELNF NLEF TEIC VNTI LYWV FARK GNPD FVEL LLKK TKDY VQDR SCSL ALIW RTFT PVYC PSPL SGIT PLLY VAQT RQSN ILKI LLQY GILE REKN
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
4933436O18Rik, ASB17
Positive Sample:
LNCaP, Rat testis
Calculated MW:
34kDa
Observed MW:
34kDa
Predicted to be involved in intracellular signal transduction. Orthologous to human ASB17 (ankyrin repeat and SOCS box containing 17).
Purification Method
Affinity purification
Gene ID
66772
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from LNCaP cells, using ASB17 Rabbit pAb (CAB20238) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Western blot analysis of lysates from Rat testis, using ASB17 Rabbit pAb (CAB20238) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
