The ATP4B Monoclonal Antibody (CAB19280) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, produced in rabbits, exhibits strong reactivity with human samples and has been validated for use in applications such as Western blotting.ATP4B, also known as H+/K+ transporting ATPase subunit beta, is essential for the functioning of the proton pump in parietal cells of the stomach. Dysregulation of ATP4B has been implicated in various gastric disorders, including gastric ulcers and gastritis.
This antibody is validated for use in IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ATP4B Monoclonal Antibody
SKU:
CAB19280
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2461
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
Hydrogen Potassium ATPase Beta, ATP4B
Cellular Localization:
Cell Membrane, Single-Pass Type Ii Membrane Protein.
Calculated MW:
33kDa
Observed MW:
Refertofigures
The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes the beta subunit of the gastric H+, K+-ATPase.
Purification Method
Affinity purification
Gene ID
496
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse stomach, using ATP4B Rabbit mAb (CAB19280) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Rat stomach using ATP4B Rabbit mAb (CAB19280) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
