The C1orf146 Antibody (CAB18804) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, has been validated for use in various research applications, including Western blotting. By targeting the C1orf146 protein, this antibody allows for detection and analysis in different cell types, making it ideal for investigations in molecular biology and disease pathology.C1orf146 is a relatively unknown protein with limited research available, making it a promising target for exploration in various fields, such as cancer biology and neurodegenerative diseases.
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
C1orf146 Antibody
SKU:
CAB18804
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SCRE, Spo16, C1orf146
Positive Sample:
Rat testis
Calculated MW:
21kDa
Observed MW:
20kDa
Involved in reciprocal meiotic recombination and synaptonemal complex assembly. Located in chromosome. Is expressed in ovary; spermatid; spermatocyte; spermatogonium; and testis. Orthologous to human C1orf146 (chromosome 1 open reading frame 146).
Purification Method
Affinity purification
Gene ID
76421
RRID
AB_2862439
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Rat testis, using C1orf146 Rabbit pAb (CAB18804) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunofluorescence analysis of paraffin-embedded rat testis using C1orf146 Rabbit pAb (CAB18804) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of paraffin-embedded mouse testis using C1orf146 Rabbit pAb (CAB18804) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
