The CDCA3 Monoclonal Antibody (CAB4921) is a high-quality antibody developed for reliable detection and analysis of target proteins. Predicted to be involved in cell division and protein ubiquitination. Located in adherens junction.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
CDCA3 Monoclonal Antibody
SKU:
CAB4921
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
ARC2135
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:500 - 1:1000
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GRCC8, TOME1, TOME-1, CDCA3
Positive Sample:
K-562
Cellular Localization:
Adherens Junction, Cytosol.
Calculated MW:
29kDa
Observed MW:
29kDa
Predicted to be involved in cell division and protein ubiquitination. Located in adherens junction.
Purification Method
Affinity purification
Gene ID
83461
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from K-562 cells, using CDCA3 Rabbit mAb (CAB4921) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Human liver cancer using CDCA3 Rabbit mAb (CAB4921) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
