The Cytokeratin 12 (KRT12) Monoclonal Antibody (CAB19284) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in applications such as immunohistochemistry and Western blotting.Cytokeratin 12 is a member of the intermediate filament family of proteins that are essential for maintaining the structural integrity of epithelial cells. Aberrant expression of cytokeratin 12 has been associated with various diseases, including ocular disorders and certain types of cancer.
This antibody is validated for use in IHC-P, ELISA, IF-P applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
Cytokeratin 12 (KRT12) Monoclonal Antibody
SKU:
CAB19284
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Clone Number:
ARC2465
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
K12, MECD1, Cytokeratin 12 (KRT12)
Cellular Localization:
Cytoskeleton, Cytosol, Extracellular Exosome.
Calculated MW:
54kDa
Observed MW:
Refertofigures
KRT12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. Mutations in this gene lead to Meesmann corneal dystrophy.
Purification Method
Affinity purification
Gene ID
3859
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Immunohistochemistry analysis of paraffin-embedded Rat cornea using Cytokeratin 12 (KRT12) (KRT12) Rabbit mAb (CAB19284) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse cornea using Cytokeratin 12 (KRT12) (KRT12) Rabbit mAb (CAB19284) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Confocal imaging of paraffin-embedded Rat eyeball tissue using Cytokeratin 12 (KRT12) Rabbit mAb (CAB19284, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
