The Desmoglein 2 Monoclonal Antibody (CAB4489) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in various applications, including immunofluorescence and immunohistochemistry.Desmoglein 2 plays a crucial role in maintaining the structural integrity of epithelial cells, particularly in the skin and heart. Dysregulation of desmoglein 2 has been implicated in autoimmune conditions such as pemphigus vulgaris, where autoantibodies target desmoglein 2, leading to loss of cell-cell adhesion and blister formation.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Desmoglein 2 Monoclonal Antibody
SKU:
CAB4489
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC1064
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HDGC, CDHF5, Desmoglein 2
Positive Sample:
HeLa, HepG2
Cellular Localization:
Cell Junction, Desmosome, Cell Membrane.
Calculated MW:
122kDa
Observed MW:
160kDa
This gene encodes a member of the desmoglein family and cadherin cell adhesion molecule superfamily of proteins. Desmogleins are calcium-binding transmembrane glycoprotein components of desmosomes, cell-cell junctions between epithelial, myocardial, and other cell types. The encoded preproprotein is proteolytically processed to generate the mature glycoprotein. This gene is present in a gene cluster with other desmoglein gene family members on chromosome 18. Mutations in this gene have been associated with arrhythmogenic right ventricular dysplasia, familial, 10.
Purification Method
Affinity purification
Gene ID
1829
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Desmoglein 2 Rabbit mAb (CAB4489) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
