The Digoxin Monoclonal Antibody (CAB20267) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, produced in rabbits, is reactive with digoxin in various samples and is validated for use in applications such as ELISA and immunohistochemistry.Digoxin is a cardiac glycoside that works by increasing the force of contraction of the heart muscle, making it an important medication for managing heart failure and certain arrhythmias.
This antibody is validated for use in ELISA, DB applications and has demonstrated reactivity against Species independent samples.
Product Name:
Digoxin Monoclonal Antibody
SKU:
CAB20267
Size:
20μL, 100μL
Reactivity:
Species independent
Clone Number:
ARC50289
Conjugate:
Unconjugated
Immunogen:
Chemical compounds corresponding to Digoxigenin/Digoxin.
Sequence:
Digo xin conj ugat ed t o KL H
Tested Applications:
ELISADB
Recommended Dilution:
DB
1:500 - 1:3000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Observed MW:
Refertofigures
Digoxin is a cardiac glycoside. Digoxin inhibits the sodium potassium adenosine triphosphatase (ATPase) pump, thereby increasing intracellular calcium and enhancing cardiac contractility. This agent also acts directly on the atrioventricular node to suppress conduction, thereby slowing conduction velocity. Apparently due to its effects on intracellular calcium concentrations, digoxin induces apoptosis of tumor cells via a pathway involving mitochondrial cytochrome c, caspases 8 and 3.
Purification Method
Affinity purification
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
The Anti-Digoxigenin/Digoxin Rabbit mAb (CAB20267) are tested in Dot Blot against digoxin labelled oligonucleotide(Dig-1，Dig-2 and Dig-3) and unlabelled oligonucleotide(Dig-4) Dig-1 :5'AGCTAAC/iDigdT/ACTAGCT(Biotin)3' Dig-2 :5'(Digoxin)AGCTAACTACTAGCT(Biotin)3' Dig-3 :5'(Biotin)AGCTAACTACTAGCT(Digoxin)3' Dig-4 :5'AGCTAACTACTAGCT(Biotin)3'
The Anti-Digoxigenin/Digoxin Rabbit mAb (CAB20267) are tested in ELISA against digoxin labelled oligonucleotide(Dig-1，Dig-2 and Dig-3) and unlabelled oligonucleotide(Dig-4) Dig-1 :5'AGCTAAC/iDigdT/ACTAGCT(Biotin)3' Dig-2 :5'(Digoxin)AGCTAACTACTAGCT(Biotin)3' Dig-3 :5'(Biotin)AGCTAACTACTAGCT(Digoxin)3' Dig-4 :5'AGCTAACTACTAGCT(Biotin)3'
