The eEF2 Monoclonal Antibody (CAB9721) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is raised in rabbits and has been validated for use in Western blot applications. It is highly reactive with human samples, enabling accurate detection and analysis of EEF2 in various cell types.EEF2, also known as eukaryotic elongation factor 2, is essential for the elongation step of protein synthesis. Dysregulation of EEF2 has been linked to various diseases, including cancer and neurodegenerative disorders. Therefore, studying the expression and activity of EEF2 is crucial for understanding its role in these conditions and developing potential therapeutic strategies.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
eEF2 Monoclonal Antibody
SKU:
CAB9721
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1717
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
EF2, EF-2, EEF-2, SCA26, eEF2
Positive Sample:
HeLa, NIH/3T3, Jurkat, Mouse liver, Rat spleen
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
95kDa
Observed MW:
95kDa
This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation.
Purification Method
Affinity purification
Gene ID
1938
RRID
AB_2863764
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using eEF2 Rabbit mAb (CAB9721) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Western blot analysis of lysates from Rat spleen, using eEF2 Rabbit mAb (CAB9721) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunofluorescence analysis of C6 cells using eEF2 Rabbit mAb (CAB9721) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using eEF2 Rabbit mAb (CAB9721) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
