The HCoV-HKU1 Spike S1 Antibody (CAB20613) is a high-quality antibody developed for reliable detection and analysis of target proteins. [Spike protein S1]: attaches the virion to the cell membrane by interacting with host receptor, initiating the infection.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against HCoV-HKU1 samples.
Product Name:
HCoV-HKU1 Spike S1 Antibody
SKU:
CAB20613
Size:
100μL, 20μL
Reactivity:
HCoV-HKU1
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:100 - 1:500
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Sample:
Human coronavirus HKU1 (isolate N5) (HCoV-HKU1) Spike Protein (S1+S2 ECDHis Tag)
Calculated MW:
152kDa
Observed MW:
75kDa/110kDa/145kDa
[Spike protein S1]: attaches the virion to the cell membrane by interacting with host receptor, initiating the infection.
Purification Method
Affinity purification
Gene ID
3200426
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Human coronavirus HKU1 (isolate N5) (HCoV-HKU1) Spike Protein (S1+S2 ECDHis Tag), using HCoV-HKU1 Spike S1 Rabbit pAb (CAB20613) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
