The HLA DMB Monoclonal Antibody (CAB0948) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, demonstrates high reactivity with human samples and has been validated for use in various applications, including Western blotting.HLA-DMB is a critical component of the major histocompatibility complex (MHC) class II molecules, which are essential for the adaptive immune response. By binding specifically to HLA-DMB, this antibody enables precise detection and analysis of this protein in a variety of cell types.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
HLA DMB Monoclonal Antibody
SKU:
CAB0948
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC2555
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RING7, D6S221E, HLA-DMB
Positive Sample:
HT-29, Raji
Cellular Localization:
Late Endosome Membrane, Lysosome Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
29kDa
Observed MW:
29kDa
HLA-DMB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta (DMB) chain, both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP (class II-associated invariant chain peptide) molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail.
Purification Method
Affinity purification
Gene ID
3109
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using HLA-DMB Rabbit mAb (CAB0948) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
