Description
| Product Name: | Anti-Human CD11b In Vivo Antibody - Ultra Low Endotoxin |
| Product Code: | IVMB0108 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | ICRF44 |
| Protein: | CD11b |
| Product Type: | Monoclonal Antibody |
| Synonyms: | Mac-1, Integrin αM chain, C3biR, CR3, Mo1, ITGAM |
| Isotype: | Mouse IgG1 κ |
| Reactivity: | Human |
| Immunogen: | Rheumatoid synovial cells and human monocytes. |
| Applications: | FC, IF Staining, IHC FF, PhenoCycler® |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | <0.5 EU/mg as determined by the LAL method |
| Purity: | ≥98% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | CODEX®, FC, IF Staining, IHC FF |
| Recommended Usage: | FC The suggested concentration for this ICRF44 antibody for staining cells in flow cytometry is ≤ 1 µg per 106 in a volume of 100 µl or 100µl of whole blood. Titration of the reagent is recommended for optimal performance for each application. |
| Reactivity: | Human |
| Host Species: | Mouse |
| Specificity: | Clone ICRF44 recognizes the α subunit (CD11b) of the human CD11b/CD18 complex. |
| Antigen Distribution: | CD11b is expressed on human peripheral blood lymphocytes, NK lymphocytes, monocytes, granulocytes, a subset of T-cells and macrophages. |
| Immunogen: | Rheumatoid synovial cells and human monocytes. |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | <0.5 EU/mg as determined by the LAL method |
| Purity: | ≥98% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
LFA-1α (CD11a) and CD18 are the Integrin alpha-L and beta-2 chains respectively that combine to form LFA-1, a glycoprotein and a member of the Integrin family. Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3, ICAM4 and for F11R. LFA-1 participates in the immunological synapses between CD8+ T lymphocytes and antigen-presenting cells. The absence of LFA-1α or β may induce LAD. The antigen contributes to natural killer cell cytotoxicity, and is involved in various immune phenomena such as leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. The CD11b/CD18 antigen is a heterodimeric surface glycoprotein on leukocytes and belongs to the β2 integrin family. CD11b functions as a receptor for C3bi complement, clotting factor X, fibrinogen and ICAM-1. CD11c forms an α/β heterodimeric glycoprotein (CD11c/CD18 complex) which belongs to the β2 integrin family. The complex binds fibrinogen and reportedly serves as a receptor for iC3b and ICAM-1. During inflammatory responses, it mediates cell to cell interaction and is important in both monocyte adhesion and chemotaxis.
| Technical Datasheet: | View |
| Protein: | CD11b |
| Function: | Adhesion, phagocytosis, chemotaxis, neutrophil activation |
| Ligand/Receptor: | ICAM-1(CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen, factor X |
| Research Area: | Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers |
| Mouse IgG1 Isotype Control [HKSP84] | |
|---|---|
| Clone | HKSP84 |
| Isotype | Mouse IgG1 |
| Endotoxin Level | Ultra Low Endotoxin |
| Mouse IgG1 Isotype Control [HKSP] | |
|---|---|
| Clone | HKSP |
| Isotype | Mouse IgG1 |
| Endotoxin Level | Ultra Low Endotoxin |
