![Anti-Human CD16 [3G8] In Vivo Antibody - Low Endotoxin](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/1280x1280/products/214447/569382/anti-human-cd16-3g8-in-vivo-antibody-low-endotoxin__18619.1673496666.jpg?c=2?imbypass=on "Anti-Human CD16 [3G8] In Vivo Antibody - Low Endotoxin")
Description
Anti-Human CD16 [3G8] In Vivo Antibody - Low Endotoxin
Introducing the Anti-Human CD16 [3G8] In Vivo Antibody - Low Endotoxin from Assay Genie, a highly specific monoclonal antibody designed for in vivo applications. This antibody targets the CD16 protein, a crucial receptor involved in immune response and natural killer (NK) cell activation, making it ideal for research in immunology, oncology, and related fields.
With a mouse IgG1 isotype, it ensures high purity and low endotoxin levels (<1.0 EU/mg), perfect for ELISA, flow cytometry, immunohistochemistry, and other assays. Available in various sizes, it is formulated in phosphate-buffered saline for stability and efficacy. Enhance your research with this reliable and versatile antibody. CD16, also known as FcγRIII, is a receptor found on the surface of various immune cells, including NK cells, macrophages, and some T-cells. It plays a significant role in antibody-dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of IgG antibodies, thus enabling the immune system to target and eliminate antibody-coated cells. This makes CD16 a pivotal component in bridging innate and adaptive immunity.
| Product Name: | Anti-Human CD16 [3G8] In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0112 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | 3G8 |
| Protein: | CD16 |
| Product Type: | Monoclonal Antibody |
| Synonyms: | Fc-gamma RIII, Fc Gamma RIII |
| Isotype: | Mouse IgG1 κ |
| Reactivity: | Baboon, Chimpanzee, Cynomolgus Monkey, Marmoset, Pigtailed Macaque, Rhesus Monkey, Squirrel Monkey, Human |
| Immunogen: | Human PMN cells |
| Applications: | B, CyTOF®, FC, IHC FF, IP |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | B, CyTOF®, FC, IHC FF, IP |
| Recommended Usage: | FC The suggested concentration for this 3G8 antibody for staining cells in flow cytometry is ≤ 2.0 µg per 106 in a volume of 100 µl or 100µl of whole blood. Titration of the reagent is recommended for optimal performance for each application. |
| Reactivity: | Baboon, Chimpanzee, Cynomolgus Monkey, Marmoset, Pigtailed Macaque, Rhesus Monkey, Squirrel Monkey, Human |
| Host Species: | Mouse |
| Specificity: | Clone 3G8 recognizes an epitope on human CD16. This clone also cross-reacts with non-human primate CD16. |
| Antigen Distribution: | CD16 is expressed on NK cells, activated monocytes, macrophages, neutrophils, and placental trophoblasts. |
| Immunogen: | Human PMN cells |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
CD16 antibody, 3G8, recognizes human CD16, also known as low-affinity IgG receptor III (FcγRIII). There are two distinct forms of CD16, CD16a (FcγRIIIa) and CD16b (FcγRIIIb). CD16a is a 50-65 kDa heterooligomeric polypeptide-anchored transmembrane protein expressed by NK cells, macrophages, and subsets of monocytes1. CD16b is a 48 kDa monomeric glycosylphosphatidylinositol (GPI)-anchored protein expressed on neutrophils1. Upon binding to the Fc portion of IgG or IgG-antigen complex, both CD16 isoforms induce signaling cascades resulting in multiple functions, including phagocytosis, cytokine release, proliferation, degranulation, and antibody dependent cell-mediated cytotoxicity (ADCC)2.
| Technical Datasheet: | View |
| Protein: | CD16 |
| Function: | Low affinity IgG Fc receptor, phagocytosis, ADCC |
| Ligand/Receptor: | Aggregated IgG, IgG-antigen complex |
| Research Area: | Cell Biology, Immunology |
| Mouse IgG1 Isotype Control [HKSP] | |
|---|---|
| Clone | HKSP |
| Isotype | Mouse IgG1 |
| Endotoxin Level | Low Endotoxin |
| Mouse IgG1 Isotype Control [HKSP84] | |
|---|---|
| Clone | HKSP84 |
| Isotype | Mouse IgG1 |
| Endotoxin Level | Low Endotoxin |
![Anti-Human CD16 [3G8] In Vivo Antibody - Low Endotoxin](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/608x608/products/214447/569382/anti-human-cd16-3g8-in-vivo-antibody-low-endotoxin__18619.1673496666.jpg?c=2 "Anti-Human CD16 [3G8] In Vivo Antibody - Low Endotoxin")
![Anti-Human CD16 [3G8] In Vivo Antibody - Ultra Low Endotoxin](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/214448/569335/anti-human-cd16-3g8-in-vivo-antibody-ultra-low-endotoxin__93977.1673496540.jpg?c=2 "Anti-Human CD16 [3G8] In Vivo Antibody - Ultra Low Endotoxin")
