The LORICRIN Polyclonal Antibody (CAB21039) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is validated for use in various applications, including immunofluorescence and immunohistochemistry.Loricrin is a key component of the skin's structure, contributing to its strength and ability to protect against environmental stressors. Research on loricrin is important for understanding skin diseases, such as ichthyosis and psoriasis, where mutations in the loricrin gene can lead to abnormalities in skin structure and function.
This antibody is validated for use in WB, IF/ICC, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
LORICRIN Polyclonal Antibody
SKU:
CAB21039
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
LOR, LORICRIN
Positive Sample:
Mouse skin, Rat skin
Cellular Localization:
Cytoplasm, Cytosol, Nucleoplasm.
Calculated MW:
26kDa
Observed MW:
26kDa
This gene encodes loricrin, a major protein component of the cornified cell envelope found in terminally differentiated epidermal cells. Mutations in this gene are associated with Vohwinkel's syndrome and progressive symmetric erythrokeratoderma, both inherited skin diseases.
Purification Method
Affinity purification
Gene ID
4014
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using LORICRIN Rabbit pAb (CAB21039) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Immunofluorescence analysis of A431 cells using LORICRIN Rabbit pAb (CAB21039) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of paraffin-embedded mouse skin using LORICRIN Rabbit pAb (CAB21039) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
