The LSD2/KDM1B/AOF1 Monoclonal Antibody (CAB19820) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot experiments. By binding to the LSD2/KDM1B/AOF1 protein, this antibody enables precise detection and analysis in a variety of cell types, making it an excellent choice for studies in epigenetics and cancer research.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
LSD2/KDM1B/AOF1 Monoclonal Antibody
SKU:
CAB19820
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2347
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
AOF1, LSD2, C6orf193, LSD2/KDM1B/AOF1
Positive Sample:
HeLa, HCT 116, NIH/3T3, PC-12, C6
Cellular Localization:
Nucleoplasm, Nucleus.
Calculated MW:
92kDa
Observed MW:
92kDa
Flavin-dependent histone demethylases, such as KDM1B, regulate histone lysine methylation, an epigenetic mark that regulates gene expression and chromatin function (Karytinos et al., 2009 [PubMed 19407342]).
Purification Method
Affinity purification
Gene ID
221656
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using LSD2/KDM1B/AOF1 Rabbit mAb (CAB19820) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of various lysates using LSD2/KDM1B/AOF1 Rabbit mAb (CAB19820) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90 s.
