Description
Anti-Mouse CD28 In Vivo Antibody - Low Endotoxin
Introducing the Anti-Mouse CD28 In Vivo Antibody - Low Endotoxin from Assay Genie, a highly specific monoclonal antibody designed for in vivo applications. This antibody targets the CD28 protein, an essential molecule involved in the co-stimulation and activation of T-cells, making it invaluable for research in immunology and related fields. With a rat IgG2a isotype, it guarantees high purity and low endotoxin levels (<1.0 EU/mg), perfect for ELISA, flow cytometry, functional assays, and other experimental setups.
Available in various sizes, it is formulated in phosphate-buffered saline for optimal stability and efficacy. Enhance your research with this robust and versatile antibody. CD28 is a critical receptor on the surface of T-cells and plays a pivotal role in their activation, survival, and proliferation. It interacts with B7 molecules on antigen-presenting cells, providing necessary co-stimulatory signals for T-cell activation and function, thereby contributing significantly to the adaptive immune response.
| Product Name: | Anti-Mouse CD28 In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0056 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | 37.51 |
| Protein: | CD28 |
| Product Type: | Monoclonal Antibody |
| Synonyms: | MGC138290, Tp44, T44 |
| Isotype: | Syrian Hamster IgG |
| Reactivity: | Mouse |
| Immunogen: | C57BL/6 mouse T-cell lymphoma EL-4 |
| Applications: | B, Costim, CyTOF®, FC, IHC FF, IP, WB |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | B, Costim, CyTOF®, FC, IHC FF, IP, WB |
| Recommended Usage: | FC The suggested concentration for this 37.51 antibody for staining cells in flow cytometry is ≤ 1.0 µg per 106 in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application. WB The suggested concentration for this 37.51 antibody for use in western blotting is 1-10 µg/ml. |
| Reactivity: | Mouse |
| Host Species: | Syrian Hamster |
| Specificity: | Clone 37.51 recognizes an epitope on mouse CD28. |
| Antigen Distribution: | CD28 is expressed on thymocytes, CD4+, CD8+ peripheral T cells, NK cells. |
| Immunogen: | C57BL/6 mouse T-cell lymphoma EL-4 |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
CD28 is a 44 kD glycoprotein and a member of the Ig superfamily. In its capacity as a costimulatory receptor, CD28 produces co-stimulatory signals necessary for T cell activation and survival. CD28 is the only B7 receptor constitutively expressed on naive T cells. Without having this CD28/B7 interaction, T cells will fail to respond to their specific antigen when coming in contact with an MHC/antigen complex and thus, are said to be anergic.
| Technical Datasheet: | View |
| Protein: | CD28 |
| Function: | Costimulates T and NK cells |
| Ligand/Receptor: | CD80 (B7-1), CD86 (B7-2) |
| Research Area: | Costimulatory Molecules, Immunology |
