Description
Anti-Mouse CD28 In Vivo Antibody - Ultra Low Endotoxin
Introducing the Anti-Mouse CD28 In Vivo Antibody - Ultra Low Endotoxin from Assay Genie, a highly specific monoclonal antibody tailored for in vivo applications. This antibody targets the CD28 molecule, an essential co-stimulatory protein in T-cell activation, making it ideal for research in immunology and related fields. Featuring a mouse IgG subclass, it ensures exceptional purity and ultra-low endotoxin levels (<0.1 EU/mg), perfect for assays such as ELISA, flow cytometry, and immunohistochemistry. Available in various concentrations, it is formulated in phosphate-buffered saline for optimal stability and performance.
Enhance your research endeavors with this reliable and highly effective antibody. CD28 is a vital receptor found on the surface of T-cells, playing a crucial role in the adaptive immune response. It functions by binding to its ligands CD80 and CD86 on antigen-presenting cells, thereby providing necessary co-stimulatory signals for T-cell activation and survival. This critical interaction underscores the importance of CD28 in maintaining a robust and balanced immune system.
| Product Name: | Anti-Mouse CD28 In Vivo Antibody - Ultra Low Endotoxin |
| Product Code: | IVMB0057 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | 37.51 |
| Protein: | CD28 |
| Product Type: | Monoclonal Antibody |
| Synonyms: | MGC138290, Tp44 |
| Isotype: | Syrian Hamster IgG |
| Reactivity: | Mouse |
| Immunogen: | C57BL/6 mouse T-cell lymphoma EL-4 |
| Applications: | B, Costim, CyTOF®, FA, IP, WB |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | <0.5 EU/mg as determined by the LAL method |
| Purity: | ≥98% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | B, Costim, CyTOF®, FA, IP, WB |
| Recommended Usage: | FC The suggested concentration for this 37.51 antibody for staining cells in flow cytometry is ≤ 1.0 µg per 106 in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application. WB The suggested concentration for this 37.51 antibody for use in western blotting is 1-10 µg/ml. |
| Reactivity: | Mouse |
| Host Species: | Syrian Hamster |
| Specificity: | Clone 37.51 recognizes an epitope on mouse CD28. |
| Antigen Distribution: | CD28 is expressed on thymocytes, CD4+, CD8+ peripheral T cells, NK cells. |
| Immunogen: | C57BL/6 mouse T-cell lymphoma EL-4 |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | <0.5 EU/mg as determined by the LAL method |
| Purity: | ≥98% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
CD28 is a 44 kD glycoprotein and a member of the Ig superfamily. In its capacity as a costimulatory receptor, CD28 produces co-stimulatory signals necessary for T cell activation and survival. CD28 is the only B7 receptor constitutively expressed on naive T cells. Without having this CD28/B7 interaction, T cells will fail to respond to their specific antigen when coming in contact with an MHC/antigen complex and thus, are said to be anergic.
| Technical Datasheet: | View |
| Protein: | CD28 |
| Ligand/Receptor: | CD80 (B7-1), CD86 (B7-2) |
| Research Area: | Costimulatory Molecules, Immunology |
