Anti-Mouse CD366 (Tim-3) In Vivo Antibody - Ultra Low Endotoxin
Introducing the Anti-Mouse CD366 [TIM-3] In Vivo Antibody - Ultra Low Endotoxin from Assay Genie, a highly specific monoclonal antibody designed for in vivo applications. This antibody targets the CD366 (also known as TIM-3) protein, which plays an integral role in regulating immune responses, making it an excellent tool for research in immunology and related fields. With a rat IgG2a isotype, it ensures exceptionally high purity and ultra low endotoxin levels (<0.1 EU/mg), making it perfectly suited for applications like ELISA, flow cytometry, immunohistochemistry, and other assays. Available in various sizes, this antibody is formulated in phosphate-buffered saline for maximum stability and efficacy.
Elevate your research capabilities with this dependable and adaptable antibody. CD366 (TIM-3) is a type I membrane protein predominantly expressed on the surface of Th1 cells, as well as on other immune cells such as dendritic cells, monocytes, and macrophages. It is a critical modulator in immune responses and is involved in the negative regulation of immune responses, helping to maintain immune homeostasis and prevent autoimmune disease. By targeting this protein, researchers can gain deeper insights into immune regulation and the pathogenesis of various immune-related disorders.
Product Name:
Anti-Mouse CD366 (Tim-3) In Vivo Antibody - Ultra Low Endotoxin
Product Code:
IVMB0390
Size:
1 mg, 5 mg, 25 mg, 50 mg, 100 mg
Clone:
RMT3-23
Protein:
Tim-3
Product Type:
Monoclonal Antibody
Synonyms:
Havcr2
Isotype:
IgG2a k
Reactivity:
Mouse
Applications:
B, FC, IF Staining, IHC, IHC FF, In Vivo, PhenoCycler®
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Endotoxin Level:
<0.5 EU/mg as determined by the LAL method
Purity:
≥98% monomer by analytical SEC â‹… >95% by SDS Page
Product Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Applications:
B, FC, IF Staining, IHC, IHC FF, In Vivo, PhenoCycler®
Reactivity:
Mouse
Host Species:
Rat
Specificity:
Clone RMT2-23 recognizes an epitope on mouse Tim-3.
Antigen Distribution:
Tim-3 is expressed on activated Th1 and Tc1 lymphocytes, CD11b+ macrophages, Tregs, NK cells, and mast cells.
Immunogen:
This antibody was produced using recombinant mouse TIM-3
Concentration:
≥ 5.0 mg/ml
Endotoxin Level:
<0.5 EU/mg as determined by the LAL method
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Purity:
≥98% monomer by analytical SEC â‹… >95% by SDS Page
Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Tim-3 antibody, clone RMT3-23, recognizes T cell immunoglobulin and mucin domain-containing protein 3 (TIM3, also known as CD366), a TIM family member of immunoregulatory proteins. Tim-3 is a 60kDa type I transmembrane protein with an extracellular immunoglobulin and mucin-like domain and a cytoplasmic tyrosine phosphorylation motif. Tim-3 is expressed by IFNg-producing Th1 CD4 and Tc1 (cytotoxic) CD8 T cells1, regulatory T cells (Tregs)2, myeloid cells3, NK cells4, and mast cells5. Tim-3 is an inhibitory molecule that limits Th1-mediated inflammatory diseases, including a model of central nervous system inflammation (experimental autoimmune encephalomyelitis, EAE)1, inflammatory bowel disease (IBD)6, and type I diabetes7. Tim-3 is also proposed to induce immunological tolerance7, promote Th1 apoptosis8, and regulate macrophage activation1. In addition, Tim-3 is upregulated on tumor-infiltrating lymphocytes, and coblockade of Tim-3 and immune checkpoint inhibitors, such as PD-1, is currently being investigated in clinical trials for the treatment of cancer9.
Protein:
Tim-3
Function:
May play a role in the development of immune responses and the development of Th1-mediated responses
Ligand/Receptor:
Putative ligand on resting CD4+ lymphocytes
Research Area:
Immunology, Inhibitory Molecules
Pathogen Testing:
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Assay Genie's Purified Functional Antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
