Description
Anti-Mouse CD70 In Vivo Antibody - Low Endotoxin
Introducing the Anti-Mouse CD70 In Vivo Antibody - Low Endotoxin from Assay Genie, a highly specific monoclonal antibody crafted for in vivo applications. This antibody targets the CD70 protein, pivotal in the regulation of immune responses, making it a valuable tool for research in immunology and related disciplines. With a rat IgG2a isotype, it guarantees high purity and low endotoxin levels (<1.0 EU/mg), making it suitable for ELISA, flow cytometry, immunohistochemistry, and other assays.
Available in various sizes, it is conveniently formulated in phosphate-buffered saline for ensured stability and efficacy. Elevate your research with this dependable and versatile antibody. CD70 is a type II transmembrane protein found primarily on activated T- and B-cells, as well as on mature dendritic cells. It plays a significant role in the immune system by enhancing the proliferation and survival of T-cells and B-cells, through interaction with the CD27 receptor.
| Product Name: | Anti-Mouse CD70 In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0170 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | TAN1-7 |
| Protein: | CD70 |
| Product Type: | Monoclonal Antibody |
| Synonyms: | CD27 Ligand, TNFSF7, Tumor Necrosis Factor Ligand 8A, CD27L, CD70 Antigen, CD27LG, Surface Antigen CD70, CD70 Molecule, Ki-24 Antigen, CD70 Antigen, TNLG8A |
| Isotype: | Mouse IgG2a κ |
| Reactivity: | Mouse |
| Immunogen: | Recombinant mouse CD70 (extracellular domain, aa residues 41-195) |
| Applications: | B, ELISA, FA, FC, IF |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | B, ELISA, FA, FC, IF |
| Recommended Usage: | FC ELISA |
| Reactivity: | Mouse |
| Host Species: | Mouse |
| Specificity: | Clone TAN1-7 monoclonal antibody recognizes a direct epitope on mouse CD70 and capable of blocking the CD70-CD27 interaction involved in B and T cell activation. |
| Antigen Distribution: | CD70 is expressed on activated T cells, B cells, and dendritic cells. |
| Immunogen: | Recombinant mouse CD70 (extracellular domain, aa residues 41-195) |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
CD70 antibody, TAN1-7, recognizes CD70, a type II membrane protein and member of the TNF family. CD70 is the ligand for the T cell costimulatory receptor CD27 (also known as TNFRSF27)1 and is expressed on activated T cells, B cells, and dendritic cells (DCs)1,2. Ligation of CD27 on T cells with CD70 on DCs provides costimulatory signals and promotes CD4 and CD8 T cell activation3, differentiation4, and survival5,6. CD70 also regulates B cell activation and antibody production7 and enhances natural killer (NK) cell survival and function8. CD70 is constitutively expressed in several hematological and solid tumors and increases the proliferation and survival of tumor cells and regulatory T cells (Tregs), suggesting it may be an effective therapeutic target9-13. Increased levels of CD70 are also associated with autoimmune disorders, including rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE)14,15.
| Technical Datasheet: | View |
| Protein: | CD70 |
| Research Area: | Costimulatory Molecules, Immunology |
| Mouse IgG2a Isotype Control | |
|---|---|
| Clone | C1.18.4 |
| Isotype | Mouse IgG2a |
| Endotoxin Level | Low Endotoxin |
