Description
Anti-Mouse CTLA-4 (CD152) In Vivo Antibody - Ultra Low Endotoxin
Introducing the Anti-Mouse CTLA-4 (CD152) In Vivo Antibody - Ultra Low Endotoxin from Assay Genie, a highly specific monoclonal antibody designed for rigorous in vivo applications.
This antibody targets the CTLA-4 protein, a crucial negative regulator of immune responses, making it ideal for research in immunology, cancer, and related fields. With a rat IgG2b kappa isotype, it ensures exceptional purity and ultra-low endotoxin levels (<0.1 EU/mg), perfect for ELISA, flow cytometry, immunohistochemistry, and other biological assays. Available in various sizes, it is formulated in phosphate-buffered saline to maintain stability and efficacy. Elevate your research with this dependable and versatile antibody. CTLA-4, also known as CD152, is an immune checkpoint receptor expressed on T cells. It plays a pivotal role in downregulating immune responses by competing with CD28 for binding to B7 molecules (CD80/CD86) on antigen-presenting cells. By transmitting an inhibitory signal to T cells, CTLA-4 is crucial in maintaining immune homeostasis and preventing autoimmunity. This makes it a significant focus of study in therapeutic interventions for autoimmune diseases, cancer immunotherapy, and transplant biology.
| Product Name: | Anti-Mouse CTLA-4 (CD152) In Vivo Antibody - Ultra Low Endotoxin |
| Product Code: | IVMB0050 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | UC10-4F10-11 |
| Protein: | CTLA-4 |
| Product Type: | Monoclonal Antibody |
| Synonyms: | CD152, Cytotoxic T Lymphocyte-Associated Antigen-4, Ly-56 |
| Isotype: | Armenian Hamster IgG1 |
| Reactivity: | Mouse |
| Immunogen: | Mouse CTLA-4 IgG2a Fusion |
| Applications: | FA, FC, IP, WB |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | <0.5 EU/mg as determined by the LAL method |
| Purity: | ≥98% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | FA, FC, IP, WB |
| Reactivity: | Mouse |
| Host Species: | Armenian Hamster |
| Specificity: | Clone UC10-4F10-11 recognizes an epitope on mouse CTLA-4. |
| Antigen Distribution: | CTLA-4 is expressed on activated T and B lymphocytes. |
| Immunogen: | Mouse CTLA-4 IgG2a Fusion |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | <0.5 EU/mg as determined by the LAL method |
| Purity: | ≥98% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
CTLA-4 is a 33 kD member of the Ig superfamily similar to CD28 in amino acid sequence, structure, and genomic organization. CTLA-4 is a protein receptor that functions as an immune checkpoint and downregulates immune responses. It is involved in the development of protective immunity and thymocyte regulation, in addition to the induction and maintenance of immunological tolerance. CTLA-4 has therapeutic potential both as an agonist to reduce immune activity, and an antagonist to increase immune activity.
| Technical Datasheet: | View |
| Protein: | CTLA-4 |
| Ligand/Receptor: | CD80 (B7-1), CD86 (B7-2) |
| Research Area: | Immunology |
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