Description
| Product Name: | Anti-Mouse H-2Db (Clone B22/249) In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0257 |
| Size: | 1 mg, 5 mg, 25 mg, 50 mg, 100 mg |
| Clone: | B22/249 |
| Product Type: | Hybridoma Monoclonal Antibody |
| Synonyms: | H-2D; H2-D; H2-D1 |
| Isotype: | Mouse IgG2a k |
| Reactivity: | Mouse |
| Applications: | Cytotoxicity Assay, In vitro depletion, IP |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. |
| Endotoxin Level: | ≤ 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% by SDS Page â‹… ≥95% monomer by analytical SEC |
| Product Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using In vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Applications: | Cytotoxicity Assay, In vitro depletion, IP |
| Reactivity: | Mouse |
| Host Species: | Mouse |
| Specificity: | B22.249.R1 activity is directed against mouse H-2Db. |
| Antigen Distribution: | H-2Db is expressed on the cell surface of lymphocytes. |
| Immunogen: | Mouse H-2Db |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | ≤ 1.0 EU/mg as determined by the LAL method |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. |
| Purity: | ≥95% by SDS Page â‹… ≥95% monomer by analytical SEC |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using In vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -70°C. Avoid Repeated Freeze Thaw Cycles. |
| Ligand/Receptor: | CD3/TCR, CD8 |
| Research Area: | Immunology |
| State of Matter: | Liquid |
| Shipping: | 2-8°C |
| NCBI Gene Bank ID: | 14964 |
