![Anti-Mouse H-2Kb (MHC Class I) [Y-3] In Vivo Antibody - Ultra Low Endotoxin](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/1280x1280/products/214345/569421/anti-mouse-h-2kb-mhc-class-i-y-3-in-vivo-antibody-ultra-low-endotoxin__09050.1770917038.jpg?c=2?imbypass=on "Anti-Mouse H-2Kb (MHC Class I) [Y-3] In Vivo Antibody - Ultra Low Endotoxin")
Description
Anti-Mouse H-2Kb (MHC Class I) [Y-3] In Vivo Antibody - Ultra Low Endotoxin
Introducing the Anti-Mouse H-2Kb MHC Class I [Y-3] In Vivo Antibody - Ultra Low Endotoxin from Assay Genie, a high-specificity monoclonal antibody tailored for in vivo research applications.
This antibody targets the H-2Kb MHC Class I protein, a critical element in immune response regulation, making it indispensable for studies in immunology and related disciplines. Featuring a rat IgG2a isotype, it ensures exceptional purity and ultra-low endotoxin levels (<0.01 EU/µg), suitable for ELISA, flow cytometry, immunoprecipitation, and other assays. Available in multiple sizes, this antibody is formulated in phosphate-buffered saline for maximal stability and performance. Elevate your research with this dependable and versatile antibody. H-2Kb MHC Class I molecules are essential components of the immune system, predominantly expressed on the surface of nucleated cells. They play a pivotal role in presenting endogenous antigens to CD8+ T cells, facilitating the immune system's ability to recognize and respond to intracellular pathogens and abnormal cells. This antibody enables researchers to effectively study antigen presentation, immune response modulation, and various other aspects of immunological function in murine models.
| Product Name: | Anti-Mouse H-2Kb (MHC Class I) [Y-3] In Vivo Antibody - Ultra Low Endotoxin |
| Product Code: | IVMB0010 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | Y-3 |
| Protein: | MHC Class I |
| Product Type: | Monoclonal Antibody |
| Synonyms: | H-2K, H-2 class I histocompatibility antigen, K-B alpha chain, H-2K(B), H-2K(K), H-2K(Q), H-2K(R), H-2K(S), Beta-2-microglobulin |
| Isotype: | Mouse IgG2b κ |
| Reactivity: | Mouse |
| Immunogen: | Con A stimulated spleen cells from BALB.B mice |
| Applications: | FA, ICC, IP, WB |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | <0.5 EU/mg as determined by the LAL method |
| Purity: | ≥98% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | FA, ICC, IP, WB |
| Reactivity: | Mouse |
| Host Species: | Mouse |
| Specificity: | Clone Y-3 recognizes an epitope on mouse MHC class I H-2K haplotypes b, k, q, r, s, but not d. |
| Antigen Distribution: | H-2K is ubiquitously expressed on nucleated cells. |
| Immunogen: | Con A stimulated spleen cells from BALB.B mice |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | <0.5 EU/mg as determined by the LAL method |
| Purity: | ≥98% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
H-2K antibody, clone Y-3, recognizes the major histocompatibility complex (MHC) class I H-2K haplotypes b, k, q, r, s, but not d. MHC class I is ubiquitously expressed on the cell surface of nucleated cells and consists of a 45-kDa type I transmembrane glycoprotein (α-chain or heavy chain) and a 12-kDa soluble protein (β2-microglobulin, β2M)1,2. The α-chain consists of three domains (α1, α2, and α3)3. α1 and α2 form the closed antigen-binding groove and bind to 8-10 aa peptides derived from cytosolic antigens4-6. β2M noncovalently associates with α3, which is essential for MHC stability. H-2K plays a critical role in the adaptive immune response by presenting endogenous antigens to cytotoxic CD8 T cells. MHC class I molecules can also present exogenous antigens to CD8 T cells via a process known as cross-presentation7. The T cell receptor (TCR)/CD3 complex of CD8 T cells interacts with peptide-MHC class I, which induces CD8 T cell activation and subsequent cell-killing. CD8 molecules also bind to MHC class I, which helps augment TCR signaling8. In contrast to CD8 T cells, MHC class I is an inhibitory ligand for natural killer (NK) cells, promoting self tolerance9. MHC class I also contributes to the positive selection of CD8 T cells and NK cell specificity10,11.
| Technical Datasheet: | View |
| Protein: | MHC Class I |
| Ligand/Receptor: | CD3/TCR, CD4 |
| Research Area: | Immunology, Innate Immunity |
| Mouse IgG2b Isotype Control | |
|---|---|
| Clone | MPC-11 |
| Isotype | Mouse IgG2b |
| Endotoxin Level | Ultra Low Endotoxin |
![Anti-Mouse H-2Kb (MHC Class I) [Y-3] In Vivo Antibody - Ultra Low Endotoxin](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/608x608/products/214345/569421/anti-mouse-h-2kb-mhc-class-i-y-3-in-vivo-antibody-ultra-low-endotoxin__09050.1770917038.jpg?c=2 "Anti-Mouse H-2Kb (MHC Class I) [Y-3] In Vivo Antibody - Ultra Low Endotoxin")
![Anti-Mouse H-2Kb (MHC Class I) [Y-3] In Vivo Antibody - Low Endotoxin](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/214344/569467/anti-mouse-h-2kb-mhc-class-i-y-3-in-vivo-antibody-low-endotoxin__11576.1770915296.jpg?c=2 "Anti-Mouse H-2Kb (MHC Class I) [Y-3] In Vivo Antibody - Low Endotoxin")
