The Phospho-CGAS-S420 Antibody (CABP1228) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, raised in rabbits, is highly specific for detecting phosphorylation at serine 420 of the cGAS protein in human samples. Validated for use in Western blot applications, this antibody allows for precise detection and analysis of phospho-cGAS, providing insights into the regulation of innate immune signaling pathways.Phosphorylation of cGAS at serine 420 has been implicated in the activation of the cGAS-STING pathway, leading to the production of type I interferons and inflammatory cytokines in response to DNA damage or pathogen invasion.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
Phospho-CGAS-S420 Antibody
SKU:
CABP1228
Size:
20μL, 100μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
FCSY H
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
Mb21d1, E330016A19Rik, Phospho-CGAS-S420
Positive Sample:
293T treated with Calyculin A, C6 treated with Calyculin A
Cellular Localization:
Cytosol, Nucleoplasm, Nucleus, Plasma Membrane.
Calculated MW:
58kDa
Observed MW:
62kDa
The protein encoded by this gene is a DNA binding cytosolic protein that catalyzes the synthesis of cyclic guanosine monophosphate-adenosine monophosphate (cGAMP) after sensing the presence of DNA in the cytoplasm. cGAMP binds another protein, Stimulator of interferon genes (STING), leading to the induction of interferons, and a host immune response. Reduced expression of this gene inhibits interferon induction in the presence of some viral infections. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
214763
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Phospho-CGAS-S420 Rabbit pAb (CABP1228) at 1:1000 dilution. 293T and C6 cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
