The Phospho-MCM2-S139 Antibody (CABP1192) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, raised in rabbits, specifically targets the phosphorylated form of MCM2 at serine 139, a key marker of DNA replication initiation.With its high reactivity in human samples, this antibody is ideal for use in Western blot applications, enabling researchers to detect and analyze phosphorylated MCM2 in various cell types. By targeting this specific phosphorylation site, researchers can gain insights into the regulation of DNA replication and cell cycle progression.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-MCM2-S139 Antibody
SKU:
CABP1192
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
YDSD E
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined.
Purification Method
Affinity purification
Gene ID
4171
RRID
AB_2864048
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-MCM2-S139 Rabbit pAb (CABP1192) at 1:1000 dilution. Hela cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour. HeLa cells were treated by Serum-starvation overnight at 37℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
