In a similar vein, the Anti-Phospho MLKL (S454) Antibody (CABP1173) offered by AssayGenie is a valuable tool for researchers studying programmed cell death pathways. MLKL is a key player in necroptosis, a form of programmed cell death that can contribute to inflammation and tissue damage. Phosphorylation of MLKL at residue S454 is a critical step in the activation of necroptosis.This highly specific rabbit polyclonal antibody recognizes the phosphorylated form of MLKL at S454, allowing for the detection and analysis of this important protein in various experimental settings. It has been validated for use in Western blot applications, providing researchers with a reliable tool for studying necroptosis and its role in diseases such as cancer, neurodegenerative disorders, and inflammatory conditions.
By targeting the phosphorylated form of MLKL, researchers can gain insights into the mechanisms underlying necroptosis and potentially identify new therapeutic targets for intervening in diseases where dysregulated cell death processes play a role. The Anti-Phospho MLKL (S454) Antibody (CABP1173) is a valuable asset for studies aimed at understanding and manipulating cell death pathways for therapeutic benefit.
Product Name:
Phospho-MLKL-S454 Rabbit Polyclonal Antibody
SKU:
CABP1173
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S454 of human MLKL (NP_689862.1).
Sequence:
HDPS VR
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
hMLKL; Phospho-MLKL-S454
Positive Sample:
HeLa
Cellular Localization:
Cell membrane, Cytoplasm.
Calculated MW:
54kDa
Observed MW:
54kDa
This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene.
Purification Method:
Affinity purification
Gene ID:
197259
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-MLKL-S454 Rabbit pAb (CABP1173) at 1:1000 dilution.Hela cells were treated by ATP(5 mM) at 30℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
