The Phospho-STING/TMEM173-S366 Polyclonal Antibody (CABP1223) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is specifically designed to target the phosphorylated form of TMEM173 at serine 366. It has been validated for use in Western blot applications and is highly reactive with human samples.TMEM173, also known as STING (Stimulator of Interferon Genes), is a key player in the innate immune response to viral and bacterial infections. Phosphorylation of TMEM173 at serine 366 is a crucial event in its activation, leading to the production of type I interferons and other pro-inflammatory cytokines.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-STING/TMEM173-S366 Polyclonal Antibody
SKU:
CABP1223
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
Email for sequence
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Autophagosome, Cytosol, Endosome, Mitochondrial Outer Membrane, Nucleoplasm, Perinuclear Region Of Cytoplasm, Peroxisome, Plasma Membrane.
Calculated MW:
42kDa
Observed MW:
40kDa
This gene encodes a five transmembrane protein that functions as a major regulator of the innate immune response to viral and bacterial infections. The encoded protein is a pattern recognition receptor that detects cytosolic nucleic acids and transmits signals that activate type I interferon responses. The encoded protein has also been shown to play a role in apoptotic signaling by associating with type II major histocompatibility complex. Mutations in this gene are the cause of infantile-onset STING-associated vasculopathy. Alternate splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
340061
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from THP-1 cells using Phospho-STING/TMEM173-S366 Rabbit pAb (CABP1223) at 1:500 dilution. THP-1 cells were differentiation with TPA (200 nM) for 15 minutes and transfection with poly(dA:dT) (5ug/mL) for 3 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 30s.
