The Anti-Phospho-ULK1 (S467) Antibody (CABP1262) is a valuable tool for researchers studying ULK1, a key regulator of autophagy. This antibody, produced in rabbits, specifically recognizes and binds to the phosphorylated form of ULK1 at serine 467. It is highly reactive with human samples and has been validated for use in Western blotting applications.ULK1 is a critical protein involved in the initiation of autophagy, a cellular process that helps maintain cellular homeostasis by degrading damaged or unnecessary cellular components.
Phosphorylation of ULK1 at serine 467 is known to play a role in regulating its activity and function in the autophagy pathway.Researchers studying autophagy, cell biology, or diseases related to autophagy dysregulation, such as neurodegenerative disorders or cancer, will find this antibody to be a valuable asset in their research. By targeting the phosphorylated form of ULK1, researchers can gain insight into the regulatory mechanisms governing autophagy and potentially uncover new therapeutic targets for a range of diseases.
Product Name:
Phospho-ULK1-S467 Rabbit Polyclonal Antibody
SKU:
CABP1262
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic phosphorylated peptide around S467 of human ULK1 (NP_003556.2).
Enables identical protein binding activity; protein serine/threonine kinase activity; and small GTPase binding activity. Involved in several processes, including autophagosome assembly; positive regulation by symbiont of host autophagy; and protein phosphorylation. Located in autophagosome; cytosol; and phagophore assembly site membrane. Is extrinsic component of autophagosome membrane; extrinsic component of omegasome membrane; and extrinsic component of phagophore assembly site membrane. Part of Atg1/ULK1 kinase complex.
Purification Method:
Affinity purification
Gene ID:
8408
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Phospho-ULK1-S467 antibody (CABP1262) at 1:1000 dilution.HeLa and Jurkat cells were treated by Serum-starvation overnight at 37℃.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
