The RhoGAP Monoclonal Antibody (CAB2330) is a high-quality antibody developed for reliable detection and analysis of target proteins. Rho GTPase activating protein 5 negatively regulates RHO GTPases, a family which may mediate cytoskeleton changes by stimulating the hydrolysis of bound GTP. Two transcript variants encoding different isoforms have been found for this gene.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
RhoGAP Monoclonal Antibody
SKU:
CAB2330
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1916
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIPELISA
Recommended Dilution:
WB
1:500 - 1:1000
IP
0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GFI2, p190-B, RhoGAP5, p190BRhoGAP, RhoGAP
Positive Sample:
Hep G2, C2C12, Mouse brain, Rat brain
Cellular Localization:
Cytoplasm, Membrane, Peripheral Membrane Protein.
Calculated MW:
172 kDa
Observed MW:
190 kDa
Rho GTPase activating protein 5 negatively regulates RHO GTPases, a family which may mediate cytoskeleton changes by stimulating the hydrolysis of bound GTP. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
394
RRID
AB_2862996
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Hep G2 cells using RhoGAP Rabbit mAb (CAB2330) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90 s.
Western blot analysis of various lysates using RhoGAP Rabbit mAb (CAB2330) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45 s.
