The SAE1 Monoclonal Antibody (CAB0891) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific and reactive with human samples, making it ideal for use in Western blot applications. It binds to the SAE1 protein, enabling detection and analysis in various cell types.The SUMOylation pathway is involved in the post-translational modification of proteins, regulating various cellular processes such as DNA repair, gene expression, and protein stability. SAE1 is a key enzyme in this pathway, essential for the activation of SUMO proteins and subsequent conjugation to target proteins.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
SAE1 Monoclonal Antibody
SKU:
CAB0891
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC1846
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
AOS1, SUA1, UBLE1A, HSPC140, SAE1
Positive Sample:
293T, MCF7, DU145
Cellular Localization:
Nucleus.
Calculated MW:
38kDa
Observed MW:
40kDa
Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).
Purification Method
Affinity purification
Gene ID
10055
RRID
AB_2861485
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SAE1 Rabbit mAb (CAB0891) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
