Description
SARS-CoV-2 NSP10 Antibody (CAB20325)
The SARS-CoV-2 NSP10 Antibody (CAB20325) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is specifically designed to target NSP10, a non-structural protein of the virus that plays a key role in viral replication and immune evasion.Raised in rabbits, this antibody is highly reactive with SARS-CoV-2 samples and has been validated for use in various applications, including Western blotting and immunofluorescence. By binding to the NSP10 protein, researchers can detect and analyze the presence of the virus in infected cells, making it an essential tool for studying the mechanisms of SARS-CoV-2 infection and developing therapeutics.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against SARS-CoV-2 samples.
| Product Name: | SARS-CoV-2 NSP10 Antibody |
| SKU: | CAB20325 |
| Size: | 20μL, 100μL |
| Reactivity: | SARS-CoV-2 |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. | ||||||
| Sequence: | AGNA TEVP ANST VLSF CAFA VDAA KAYK DYLA SGGQ PITN CVKM LCTH TGTG QAIT VTPE ANMD QESF GGAS CCLY CRCH IDHP NPKG FCDL KGKY VQIP TTCA NDPV GFTL KNTV CTVC GMWK GYGC SCDQ LREP MLQ | ||||||
| Tested Applications: | WB IP ELISA | ||||||
| Recommended Dilution: |
|
| Positive Sample: | 293T, |
| Calculated MW: | 794kDa |
| Observed MW: | 20-25kDa |
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ ORF1ab, the largest gene, contains overlapping open reading frames that encode polyproteins PP1ab and PP1a. The polyproteins are cleaved to yield 16 nonstructural proteins, NSP1-16. Production of the longer (PP1ab) or shorter protein (PP1a) depends on a -1 ribosomal frameshifting event. The proteins, based on similarity to other coronaviruses, include the papain-like proteinase protein (NSP3), 3C-like proteinase (NSP5), RNA-dependent RNA polymerase (NSP12, RdRp), helicase (NSP13, HEL), endoRNAse (NSP15), 2'-O-Ribose-Methyltransferase (NSP16) and other nonstructural proteins. SARS-CoV-2 nonstructural proteins are responsible for viral transcription, replication, proteolytic processing, suppression of host immune responses and suppression of host gene expression. The RNA-dependent RNA polymerase is a target of antiviral therapies.
| Purification Method | Affinity purification |
| Gene ID | 43740578 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of extracts of normal 293T cells, 293T transfected with NSP10 Protein and 293T transfected with SOST Protein, using SARS-CoV-2 NSP10 Rabbit pAb (CAB20325) at 1:10000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s. |
 | Immunoprecipitation of SARS-CoV-2 NSP10 from 300 µg extracts of 293T cells transfected with SARS-CoV-2 NSP10 was performed using 2 µg of SARS-CoV-2 NSP10 Rabbit pAb (CAB20325). Rabbit IgG isotype control(AC042) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10 % of the total input.Western blot analysis of immunoprecipitates was conducted using SARS-CoV-2 NSP10 Rabbit pAb (CAB20325) at a dilution of 1:4000. |
