The SARS-CoV-2 Spike S1 Antibody (CAB20136) is a high-quality antibody developed for reliable detection and analysis of target proteins. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The spike glycoprotein is found on the outside of the virus particle and gives coronavirus viruses their crown-like appearance. This glycoprotein mediates attachment of the virus particle and entry into the host cell. S protein is an important target for vaccine development, antibody therapies and diagnostic antigen-based tests.
This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, SARS-CoV-2 samples.
Product Name:
SARS-CoV-2 Spike S1 Antibody
SKU:
CAB20136
Size:
100μL, 20μL
Reactivity:
Human, SARS-CoV-2
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCIPELISA
Recommended Dilution:
WB
1:500 - 1:1000
IF/ICC
1:100 - 1:500
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
1:20000-1:80000
Synonyms:
spike glycoprotein, SARS-CoV-2 Spike S1
Positive Sample:
293T
Calculated MW:
141kDa
Observed MW:
110kDa
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The spike glycoprotein is found on the outside of the virus particle and gives coronavirus viruses their crown-like appearance. This glycoprotein mediates attachment of the virus particle and entry into the host cell. S protein is an important target for vaccine development, antibody therapies and diagnostic antigen-based tests.
Purification Method
Affinity purification
Gene ID
43740568
RRID
AB_2862928
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from 293T cells, using SARS-CoV-2 Spike S1 Rabbit pAb (CAB20136) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunofluorescence analysis of 293T cells transfected with SARS-CoV-2 Spike S1 fusion protein (top left) and untreated 293T cells (top right) use SARS-CoV-2 Spike S1 Rabbit pAb (CAB20136) at dilution of 1:400 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immobilized Recombinant SARS-COV-2 Spike S1 Protein (RP01262LQ) at 1μg/mL (100μL/well) can bind SARS-CoV-2 Spike S1 Rabbit pAb (CAB20136) with a linear range of 0.78-50ng/mL.
