The SARS-CoV-2 Spike S2 ECD Antibody (CAB20138) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced and validated for use in various research applications including ELISA and Western blot, is highly specific and sensitive in detecting the SARS-CoV-2 spike protein.The SARS-CoV-2 spike protein is a key target for vaccine development and therapeutic interventions against COVID-19. By targeting the S2 ECD region of the spike protein, this antibody provides researchers with a valuable tool for studying the structure and function of the virus, as well as potential interactions with host cells.
This antibody is validated for use in WB, IF/ICC, IP, ELISA, DB applications and has demonstrated reactivity against Human, SARS-CoV-2 samples.
Product Name:
SARS-CoV-2 Spike S2 ECD Antibody
SKU:
CAB20138
Size:
20μL, 100μL
Reactivity:
Human, SARS-CoV-2
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
spike glycoprotein, SARS-CoV-2 Spike S2 ECD
Positive Sample:
293T
Calculated MW:
141kDa
Observed MW:
75kDa
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The spike glycoprotein is found on the outside of the virus particle and gives coronavirus viruses their crown-like appearance. This glycoprotein mediates attachment of the virus particle and entry into the host cell. S protein is an important target for vaccine development, antibody therapies and diagnostic antigen-based tests.
Purification Method
Affinity purification
Gene ID
43740568
RRID
AB_2862930
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of extracts of normal 293T cells 293T transfected with Spike S2 ECD Protein, using SARS-CoV-2 Spike S2 ECD Rabbit pAb (CAB20138) at 1:5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 1s.
Immobilized Recombinant SARS-COV2 S-ECD-PFS Protein(RP01283LQ) at 1μg/mL (100μL/well) can bind SARS-CoV-2 Spike S2 ECD Rabbit pAb (CAB20138) with a linear range of 3.12-50ng/mL.
Immunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg SARS-CoV-2 Spike S2 ECD antibody (CAB20138). Western blot was performed from the immunoprecipitate using SARS-CoV-2 Spike S2 ECD antibody (CAB20138) at a dilution of 1:5000.
