The SCAF8 Antibody (CAB19467) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, produced in rabbits, exhibits high reactivity with human samples and is suitable for use in Western blot applications. By binding specifically to the SCAF8 protein, it enables detection and analysis in various cell types, making it ideal for research in molecular biology and genetics.SCAF8, also known as SR-related CTD-associated factor 8, plays a crucial role in splicing regulation and RNA processing, influencing gene expression patterns and cellular functions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SCAF8 Antibody
SKU:
CAB19467
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RBM16, SCAF8
Positive Sample:
HeLa, Mouse brain, Rat brain
Cellular Localization:
Nucleus, Nucleus Matrix.
Calculated MW:
141kDa
Observed MW:
170kDa
Enables RNA binding activity and RNA polymerase II C-terminal domain phosphoserine binding activity. Involved in negative regulation of termination of RNA polymerase II transcription, poly(A)-coupled and positive regulation of DNA-templated transcription, elongation. Located in nucleoplasm.
Purification Method
Affinity purification
Gene ID
22828
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SCAF8 Rabbit pAb (CAB19467) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Western blot analysis of lysates from HeLa cells, using SCAF8 Rabbit pAb (CAB19467) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
