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Symmetric DiMethyl-Histone H3-R8 Polyclonal Antibody (CAB20820)

SKU:
CAB20820
Product Type:
Antibody
Antibody Type:
Secondary Antibody
Host Species:
Rabbit
Isotype:
IgG
  • Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Symmetric DiMethyl-Histone H3-R8 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
  • Western blot analysis of extracts of various cell lines, using Symmetric DiMethyl-Histone H3-R8 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
€179 - €469
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Symmetric DiMethyl-Histone H3-R8 Polyclonal Antibody
SKU:CAB20820
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:A synthetic symmetric dimethylated peptide around R8 of human histone H3 (NP_003520.1).
Sequence:QTAR K
Tested Applications:WB ChIP ELISA
Recommended Dilution:WB,1:100 - 1:500 ChIP,5μg antibody for 5μg-10μg of Chromatin
Synonyms:H3/A; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; H3C10; H3C11; H3C12; HIST1H3A; Symmetric DiMethyl-Histone H3-R8
Positive Sample:HeLa,NIH/3T3,C6,H3
Conjugate:Unconjugated
Cellular Localization:Chromosome, Nucleus.
Calculated MW:15kDa
Observed MW:17kDa

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

Purification Method:Affinity purification
Gene ID:8290/8350
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using Symmetric DiMethyl-Histone H3-R8 Rabbit pAb (CAB20820) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

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