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Symmetric DiMethyl-Histone H3-R17 Rabbit Polyclonal Antibody (CAB3152)

SKU:
CAB3152
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling
  • Epigenetics and Nuclear Signaling Antibodies 3 Anti-Symmetric DiMethyl-Histone H3-R17 Antibody CAB3152
  • Epigenetics and Nuclear Signaling Antibodies 3 Anti-Symmetric DiMethyl-Histone H3-R17 Antibody CAB3152
  • Epigenetics and Nuclear Signaling Antibodies 3 Anti-Symmetric DiMethyl-Histone H3-R17 Antibody CAB3152
  • Epigenetics and Nuclear Signaling Antibodies 3 Anti-Symmetric DiMethyl-Histone H3-R17 Antibody CAB3152
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Symmetric DiMethyl-Histone H3-R17 Rabbit Polyclonal Antibody
SKU:CAB3152
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:A synthetic symmetric dimethylated peptide around R17 of human histone H3 (NP_003520.1).
Sequence:APRK Q
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:WB,1:100 - 1:500 IF/ICC,1:50 - 1:200
Synonyms:H3/j; H3C1; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FJ; H3C10; H3C11; HIST1H3J; Symmetric DiMethyl-Histone H3-R17
Positive Sample:HeLa,NIH/3T3,C6,H3
Conjugate:Unconjugated
Cellular Localization:Chromosome, Nucleus.
Calculated MW:15kDa
Observed MW:17kDa

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.

Purification Method:Affinity purification
Gene ID:8290/8350
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using Symmetric DiMethyl-Histone H3-R17 Rabbit pAb (CAB3152) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.

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