The Synapsin II Monoclonal Antibody (CAB19542) is a high-quality antibody developed for reliable detection and analysis of target proteins. Synapsin II is known for its role in synaptic plasticity and neurotransmission, making it a key target for studies in neuroscience and neurobiology.This polyclonal antibody, produced in rabbits, offers high reactivity and specificity for detecting synapsin II in human samples, making it an essential tool for Western blot applications. By targeting the synapsin II protein, researchers can analyze its expression and localization in various neuronal cell types, aiding in the understanding of synaptic function and communication in the brain.
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Synapsin II Monoclonal Antibody
SKU:
CAB19542
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2166
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene is a member of the synapsin gene family. Synapsins encode neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. This member of the synapsin family encodes a neuron-specific phosphoprotein that selectively binds to small synaptic vesicles in the presynaptic nerve terminal. Polymorphisms in this gene are associated with abnormal presynaptic function and related neuronal disorders, including autism, epilepsy, bipolar disorder and schizophrenia. Alternative splicing of this gene results in multiple transcript variants. The tissue inhibitor of metalloproteinase 4 gene is located within an intron of this gene and is transcribed in the opposite direction.
Purification Method
Affinity purification
Gene ID
6854
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from 293T cells, using Synapsin II Rabbit mAb (CAB19542) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Confocal imaging of paraffin-embedded Mouse brain using Synapsin II Rabbit mAb (CAB19542, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
