The Timm22 Polyclonal Antibody (CAB20813) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to TIMM22, this antibody enables precise detection and analysis of the protein in diverse cell types, making it an essential component for investigations in cell biology and mitochondrial research.TIMM22 is a crucial component of the mitochondrial import machinery, facilitating the transport of nuclear-encoded proteins into the mitochondrial inner membrane.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Timm22 Polyclonal Antibody
SKU:
CAB20813
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
Tim22, Timm22
Positive Sample:
T47D, Mouse brain, Rat testis, Rat brain
Cellular Localization:
Mitochondrial Inner Membrane, Mitochondrion.
Calculated MW:
20kDa
Observed MW:
20kDa
Predicted to enable mitochondrion targeting sequence binding activity and protein transmembrane transporter activity. Predicted to be involved in protein insertion into mitochondrial inner membrane. Predicted to act upstream of or within protein transport. Located in mitochondrion. Is expressed in several structures, including alimentary system; brain; cardiovascular system; egg cylinder; and genitourinary system. Human ortholog(s) of this gene implicated in combined oxidative phosphorylation deficiency 43. Orthologous to human TIMM22 (translocase of inner mitochondrial membrane 22).
Purification Method
Affinity purification
Gene ID
56322
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Timm22 Rabbit pAb (CAB20813) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunoprecipitation analysis of 600 μg extracts of Rat brain cells using 3 μg Timm22 antibody (CAB20813). Western blot was performed from the immunoprecipitate using Timm22 antibody (CAB20813) at a dilution of 1:500.
