The ULK3 Monoclonal Antibody (CAB5959) is a high-quality antibody developed for reliable detection and analysis of target proteins. Enables protein serine/threonine kinase activity. Involved in several processes, including fibroblast activation; protein autophosphorylation; and regulation of smoothened signaling pathway. Located in cytoplasm.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
ULK3 Monoclonal Antibody
SKU:
CAB5959
Size:
100μL, 20μL
Reactivity:
Human, Mouse
Clone Number:
ARC2118
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:2000
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ULK3
Positive Sample:
293T
Cellular Localization:
Autophagosome, Ciliary Tip, Cytoplasm.
Calculated MW:
53kDa
Observed MW:
50kDa
Enables protein serine/threonine kinase activity. Involved in several processes, including fibroblast activation; protein autophosphorylation; and regulation of smoothened signaling pathway. Located in cytoplasm.
Purification Method
Affinity purification
Gene ID
25989
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from 293T cells using ULK3 Rabbit mAb (CAB5959) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 10s.
Confocal imaging of C2C12 cells using ULK3 Rabbit mAb (CAB5959, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
